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KMID : 0613820170270070796
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Journal of Life Science
2017 Volume.27 No. 7 p.796 ~ p.804
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Identification of Antioxidant Compound Derived from Methanolic Extract of Houttuynia Cordata
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Kim Hye-Ji
Hwang Hee-Sung
Park Su-Min
Kang Sung-Wook
Kim Hye-Jeong
Hong Su-Gyeong
Kim Moon-Moo
Oh Yung-Hee
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Abstract
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This study was carried out to evaluate the antioxidant effect of methanolic extract of Houttuynia cordata (HCME) and to identify a compound having antioxidant effect. The ethyl acetate fraction of HCME showed the highest antioxidant effect in organic solvent fractions. The fraction was then separated into 12 fractions by open column chromatography. Among these fractions, the fraction 10 (Fr. 10) with the highest antioxidant activity was isolated, and its antioxidant effect was evaluated by DPPH radical scavenging activity, reducing power, TBARS, cell viability, DNA oxidation and DCF fluorescence. The Fr. 10 at a 64 μg/ml showed 60% of inhibitory effect similar to that of vitamin C at 10 μg/ml, compared with blank group. The Fr. 10 at 64 μg/ml showed 264% of reducing power, compared with blank group. TBARS assay showed that the Fr. 10 at 64 μg/ml had 35.5% of inhibitory effect similar to that of vitamin E at 1,000 μg/ml, compared with blank group. The Fr. 10 above 32 μg/ml displayed cytotoxicity. However, it was observed that the Fr. 10, above 1 μg/ml reduced DNA damage. DCF fluorescence assay showed that the Fr. 10 inhibited oxidative stress by H₂O₂ in a dose dependent manner. The compound of Fr. 10 was identified to be rutin whose molecular weight is 610 by the IR and LC-MS analyses. Therefore, these results suggest that the rutin of Fr. 10 could use as a natural antioxidant for development of cosmetics and functional foods.
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KEYWORD
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Antioxidant effect, DCF fluorescence, Houttuynia cordata, rutin
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